Invitrogen

Fibronectin Monoclonal Antibody (A32)

Product Details

CSI 005-32-02

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

1:4,000

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

A32

Immunogen

Bovine corneal endothelial cells.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.12 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074422

Product Specific Information

CSI 005-32-02 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). CSI 005-32-02 cross-reacts with human fibronectin. Other species have not been tested.

CSI 005-32-02 has been successfully used in Western blotting, ELISA, Immunohistochemistry and Immunoprecipitation procedures. The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain. It can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Iodide modulates protein damage induced by the inflammation-associated heme enzyme myeloperoxidase.

Redox biology

Gamon LF,Dieterich S,Ignasiak MT,Schrameyer V,Davies MJ

CSI 005-32-02 was used in ELISA to examine the hypothesis that elevated levels of I− would decrease HOCl formation and thereby protein damage induced by a MPO/Cl−/H2O2 system, by acting as a competitive substrate.

Wed Jan 01 00:00:00 EST 2020

The fibronectin ED-A domain enhances recruitment of latent TGF-β-binding protein-1 to the fibroblast matrix.

Journal of cell science

Klingberg F,Chau G,Walraven M,Boo S,Koehler A,Chow ML,Olsen AL,Im M,Lodyga M,Wells RG,White ES,Hinz B

CSI 005-32-02 was used in Immunohistochemistry to suggest that the ED-A domain enhances association of the latent TGF-β1 by promoting weak direct binding to LTBP-1 and by enhancing heparin-mediated protein interactions through HepII in fibronectin.

Thu Mar 01 00:00:00 EST 2018

The fibronectin ED-A domain enhances recruitment of latent TGF-β-binding protein-1 to the fibroblast matrix.

Journal of cell science

Klingberg F,Chau G,Walraven M,Boo S,Koehler A,Chow ML,Olsen AL,Im M,Lodyga M,Wells RG,White ES,Hinz B

Thu Mar 01 00:00:00 EST 2018

Peroxynitrite-mediated oxidation of plasma fibronectin.

Free radical biology & medicine

Degendorfer G,Chuang CY,Kawasaki H,Hammer A,Malle E,Yamakura F,Davies MJ

CSI0053202 was used in immunohistochemistry - frozen section to study peroxynitrous acid-mediated fibronectin modifications and how they contribute to cellular dysfunction

Mon Aug 01 00:00:00 EDT 2016

Heparin-dependent regulation of fibronectin matrix conformation.

Matrix biology : journal of the International Society for Matrix Biology

Hubbard B,Buczek-Thomas JA,Nugent MA,Smith ML

CSI 005-32-02 was used in ELISA and immunohistochemistry to describe an antibody-based method for evaluating the conformation of the heparin 2 binding domain in fibronectin, and use it to determine the relative contributions of heparin and mechanical strain to the regulation of fibronectin conformation

Sat Feb 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; cumulus cell-specific fibronectin 1 transcript; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; embryo-specific fibronectin 1 transcript; ferritin L subunit; ferritin L-chain; Fibronectin; fibronectin ED-A- region; fibronectin variable region; FN; Migration stimulating factor (MSF); migration-stimulating factor

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Gene Aliases: CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF

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UniProt ID: (Human) P02751

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Entrez Gene ID: (Bovine) 280794, (Human) 2335

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Function(s)

protein binding heparin binding disordered domain specific binding protease binding integrin binding collagen binding peptidase activator activity identical protein binding mercury ion binding extracellular matrix protein extracellular matrix structural protein

Process(es)

angiogenesis acute-phase response regulation of cell shape cell activation regulation of protein phosphorylation platelet degranulation response to ischemia cell-substrate junction assembly cell adhesion calcium-independent cell-matrix adhesion positive regulation of cell proliferation glial cell migration response to wounding response to ozone positive regulation of gene expression positive regulation of peptidase activity peptide cross-linking extracellular matrix disassembly extracellular matrix organization integrin activation substrate adhesion-dependent cell spreading cellular response to vascular endothelial growth factor stimulus endodermal cell differentiation cellular response to platelet-derived growth factor stimulus wound healing negative regulation of apoptotic process positive regulation of axon extension positive regulation of fibroblast proliferation leukocyte migration positive regulation of chemotaxis response to glucocorticoid regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to mercury ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to prostaglandin E stimulus cellular response to transforming growth factor beta stimulus cellular response to BMP stimulus positive regulation of substrate-dependent cell migration, cell attachment to substrate negative regulation of transforming growth factor-beta secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Fibronectin Monoclonal Antibody (A32)

Product Details

CSI 005-32-02

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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